FT-ITC Analysis

From thermogram to thermodynamics

Better analysis. Deeper understanding.

FT-ITC Analysis is a free, open-source desktop application for processing, fitting and presenting isothermal titration calorimetry data. It combines flexible raw-data processing with single- and multiple-experiment fitting, optional global constraints, uncertainty estimation and advanced thermodynamic interpretation.

Download FT-ITC Analysis Explore the desktop app
  • Flexible processing & fitting
  • Multiple-experiment analysis
  • Publication-ready figures and reports
An ITC thermogram, fitted binding isotherm, and residual plot exported from FT-ITC Analysis
Thermogram, fitted model and parameters in one publication-ready figure.

Desktop first

Download the analysis workspace.

Work with experimental data on your own computer, revisit any processing step and save complete analyses as portable FT-ITC projects.

macOSv1.4.2 · Available now ↗Windowsv1.4.2 · Available now ↗Linuxv1.4.2 · Available now ↗

From raw data to combined results

Flexible baselinesFast integration toolsBuffer subtraction & experiment mergingMultiple-experiment fittingAdvanced analysesPublication-ready figures and reports

Research use cases

Used in real thermodynamic studies.

FT-ITC Analysis has been used to quantify binding thermodynamics and examine how molecular conformation changes an interaction.

Nat.Commun. 2024
DREB2A-Med25 binding in plant stress responses ITC characterized affinity and thermodynamic changes across DREB2A variants · Nature Communications 15, 592 · Theisen et al.
JACS 2025
Cis/trans proline selection in 14-3-3 binding ITC was combined with NMR and molecular dynamics to resolve isomer-specific binding · JACS 147, 5714-5724 · Theisen et al.

A visible workflow

Work through the experiment without losing the thread.

FT-ITC Analysis brings data import, processing, fitting, advanced analysis and figure creation into one application. Start with raw thermograms, integrated heats or an existing FT-ITC project. Adjust the workflow to the experiment and adapt to the needs of your data.

01

Open & inspect

Import data and review the experimental setup. Check instrument settings and injection details. Verify temperature and adjust concentrations.

02

Process & correct

Refine polynomial, segmented or spline baselines; estimate or adjust integration regions; and apply buffer subtraction or merge tandem experiments when needed.

03

Fit, analyse & export

Fit one experiment or analyse related datasets together, use compatible advanced analyses and create publication figures, supporting panels and complete analysis reports.

Explore flexible processing →
Abstracted FT-ITC Analysis interface showing temperature-dependent thermodynamic parameters and an experiment table
Evaluate related experiments together across temperature, with fitted thermodynamic relationships and experiment-level parameters in view.

Multiple-experiment analysis

Analyse related experiments together.

Fit several isotherms in one combined analysis. Keep parameters experiment-specific, share selected values across the series, or apply supported temperature-dependent relationships for a global fit.

See the app capabilities →
Supporting Figure workspace combining three experiment figures on a multi-panel canvas
Bring related experiments together in a supporting figure with a shared layout and panel labels.

Figures and reports

Take your results from analysis to presentation.

Create publication figures, compose multi-panel supporting figures, and assemble complete PDF reports with saved results, tables, diagnostics and your interpretation.

Explore figures and reports →

Web viewer

Share results without losing the evidence.

The online viewer opens FT-ITC project files for easy sharing and review, keeping experimental data, fits and analysis results together.

Online project viewer

Checking availability… Open a project in the browser for easy sharing and review.

Open the web viewer ↗